The 33-year-old, who celebrated her daughter Blue Ivy's third birthday on Jan 7, has posted a photograph on photo-sharing site Instagram from a family day at the beach Jan 10.
In the picture, she is seen enjoying the sand along with daughter who is also spotted playing with sand. But what sparked the rumour is the singer's increased bump covered with sand.
After the photograph was posted, fans too burst into comment mode with excitement, as they noticed the increased body size.
In another picture when Beyonce and Jay visited a Buddha temple in Cambodia, Beyonce looked very much pregnant.
One of the fan wrote: "Is B preggo???"
Another added: "Baby bump," while one user wrote: "?! Does this mean she's pregnant?"
The photograph post comes months after Beyonce's partner Jay Z in September 2014 sparked rumours the couple were expecting their second child.
Changing the song lyrics to his hit "Beach is Better", the 44-year-old producer rapped: "Cos she pregnant with another one", apparently revealing that the couple are expecting their second baby.
A few days back, Beyonce shared one of the pics from a holiday which also sparked her second pregnancy rumours as the loose fitted flowing maxi seemed to have hid her baby bump.
However, there has not been any official announcement from the couple.